Center for Fish Biology and Fishery Biotechnology

Research Group of Fish Developmental Genetics and Cell Engineering



PI: Prof. GUI Jianfang, PhD (CAS member)

Members:Li Zhou (Prof. / Dr.), Yi-Bing Zhang (Prof. / Dr.), Zhong-Wei Wang (Associate Prof. / Dr.), Yang Wang (Research Associate), Zhi Li (Research Assistant), Li-Hua Wei (Research Assistant), Bei-Bei Huang (Research Associate), and others.   

Contact Us: 

Tel: 86-27-68780707 


General Introduction: 

The Research Group of Fish Developmental Genetics and Cell Engineering concentrated on two aspects of research.  

One is to identify reproduction- and immune-related genes, thereby revealing molecular regulative mechanisms on fish meiosis and gynogenesis, sex determination and differentiation, immunity and disease resistance, and developing biotechnologies of chromosome ploidy manipulation, sex control and molecular design breeding.  

The other is to analyze the genome of polyploid fish, reveal the genetic mechanisms on economic trait improvement in aquaculture fish caused by genome or sub-genome integration, thereby exploiting cell engineering biotechnology for genetic breeding of aquaculture animals. 

Recent Publications:  


[1]  Mei, J., Li, Z., Gui, J-F., 2009. Cooperation of Mtmr8 with PI3K regulates actin filament modeling and muscle development in zebrafish. PLoS ONE 4(3): e4979, doi:10.1371/journal.pone.0004979,1-12. 

[2]  Wu, N., Yue, H.M., Chen, B., Gui, J.F., 2009. Histone H2A has a novel variant in fish oocytes. Biology of Reproduction, 81: 275-283. DOI: 10.1095/biolreprod.108.074955. 

[3]  Cao, H., Zhou, L., Zhang, Y.Z., Wei, Q.W., Chen, X.H., Gui, J.F., 2009. Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals. Molecular and Cellular Endocrinology,303: 34-42. 

[4]  Li, M.Y., Hong, N., Xu, H.Y., Yi, M.S., Li, C.M., Gui, J.F., Hong, Y.H., 2009. Medaka vasa is required for migration but not survival of primordial germ cells. Mech. Dev., 126366-381. 

[5]  Huang, W., Zhou, L., Li, Z., Gui, J.F., 2009. Expression pattern, cellular localization and promoter activity analysis of ovarian aromatase (Cyp19a1a) in protogynous hermaphrodite red-spotted grouper. Molecular and Cellular Endocrinology, 307: 224-236. 

[6]  Jiang, J., Zhang, Y.B., Li,S., Yu, F.F., Sun, F., Gui, J.F., 2009. Expression regulation and functional characterization of a novel interferon-inducible gene Gig2 and its promoter. Molecular Immunology, 46(15): 3131-3140. 

[7]  Zhao, J.G., Zhou, L., Jin, J.Y., Zhao, Z., Lan, J., Zhang, Y.B., Zhang, Q.Y., Gui, J.F., 2009. Antimicrobial activity-specific to Gram-negative bacteria and immune modulation-mediated-NF-κB and Sp1 of a medaka β-defensin. Developmental and Comparative Immunology, 33: 624-637. 

[8]  Zhong, X.P., Wang, D., Zhang, Y.B., Gui, J.F., 2009. Identification and characterization of hypoxia-induced genes in Carassius auratus blastulae embryonic cells using suppression subtractive hybridization. Comparative Biochemistry and Physiology B, 152(2): 161-170. 

[9]  Xia, J.H., Jiang, J., Shi, Y.H., Gui, J.F., 2009. Predominant expression and cellular distribution of fish Agr2 in renal collecting system. Comparative Biochemistry and Physiology B, 152(4): 397-404. 

[10] Zhao, Z., Ke, F., Shi, Y., Zhou, G.Z., Gui, J.F., Zhang, Q.Y., 2009. Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein. Virus Genes, 38: 345-352. 

[11] Liu, J.X., Zhai,Y.H., Gui, J.F., 2009. Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp. Molecular Biology Reports, 36: 1491-1496. 

[12] Liu, J.X., Zhai,Y.H., Gui, J.F., 2009. Molecular Characterization and Expression Pattern of AFPIV during embryogenesis in gibel carp(Carassiu auratus gibelio). Molecular Biology Reports, 36: 2011-2018. 

[13] Lan, J., Lei, M.G., Zhang, Y.B., Wang, J.H., Feng, X.T., Xu, D.Q., Gui J.F. and Xiong, Y.Z., 2009. Characterization of the porcine differentially expressed PDK4 gene and association with meat quality. Molecular Biology Reports, 36: 2003-2010. 

[14] Liu, J.X., Hu, B., Wang, Y., Gui, J.F., Xiao, W., 2009. Zebrafish eaf1 and eaf2/u19 mediate effective convergence and extension movements through the maintenance of wnt11 and wnt5 expression. J. Biol. Chem., 284: 16679-16692. 

[15] Li, C.J., Wei, Q.W., Zhou, L., Cao, H., Zhang, Y., Gui, J.F., 2009. Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis. Comp. Biochem. Physiol. A Mol. Integr. Physiol., 154: 127-134. 

[16] Zhu, R., Zhang, Y.B., Zhang, Q.Y., Gui, J.F., 2009.Subcellular localization and inductive expression of the dsRNA-dependent protein kinase PKR from Japanese flounder, Paralichthys olivaceus. Progress on Natural Sciences, available online. 

[17] Wu, N., Li, C.J., Gui, J.F., 2009. Molecular characterization and functional commonality of nucleophosmin/nucleoplasmin in two cyprinid fish. Biochemical Genetics, DOI: 10.1007/s10528-009-9274-y, available online. 

[18] Wang, D., Mao, H.L., Peng, J.X., Li, X.Y., Zhou, L., Gui, J.F., 2009. Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio. Progress on Natural Sciences, available online. 

[19] Wang, D., Mao, H.L., Chen, H.X., Liu, H.Q., Gui, J.F., 2009. Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations. Animal Genetics, available online. 

[20] Peng, J.X., Xie, J.L., Zhou, L., Hong, Y.H., Gui, J.F., 2009. Evolutionary conservation of Dazl genomic organization and its continuous and dynamic distribution throughout germline development in gynogenetic gibel carp. J. Exp. Zool. Part B, available online DOI: 10.1002/jez.b.21301  

[21] Shi, Y., Zhu, X.P., Yin, J.K., Zhang, Q.Y., Gui, J.F. 2009. Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides). Mol. Biol. Rep., available online 

[22] Zhou, L., Gui, J.F., 2009. Molecular mechanisms underlying sex change in hermaphroditic groupers. Fish Physiology and Biochemistry, available online. 


[23] Mei, J., Zhang,Q.Y., Li,Z., Lin,S., Gui, J.F., 2008. C1q-like inhibits p53-mediated apoptosis and controls normal hematopoiesis during zebrafish embryogenesis.Developmental Biology,319: 273-284. 

[24] Zhu, R., Zhang, Y.B., Zhang, Q.Y., Gui, J.F., 2008. Functional domains and the antiviral effect of the dsRNA-dependent protein kinase PKR from Paralichthys olivaceu. Journal of Virology, 82: 6889-6901. 

[25] Mei, J., Chen, B., Yue, H.M., Gui, J.F., 2008. Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio. Molecular and Cellular Endocrinology, 289: 67-76. 

[26] Shi, Y., Zhang, Y.B., Zhao, Z., Jiang, J., Zhang, Q.Y., Gui, J.F., 2008. Molecular characterization and subcellular localization of Carassius auratus interferon regulatory factor-1. Developmental and Comparative Immunology, 32: 134-146. 

[27] Ma, D.M., Zhu, H. P., Gui, J.F., 2008. Ectopic Six3 expression in the dragon eye goldfish. Comparative Biochemistry and Physiology B, 149: 303-313.  

[28] Li, F.B., Gui, J.F., 2008. Clonal diversity and genealogical relationships of gibel carp in four hatcheries. Animal Genetics, 39: 28-33. 

[29] Mei, J., Gui, J.F., 2008. Bioinformatic identification of genes encoding C1q-domain-containing proteins in zebrafish. Journal of Genetics and Genomics, 35: 17-24. 

[30] Zhao Z, Shi Y, Ke F, Wei S, Gui J, Zhang, Q.Y., 2008. Constitutive expression of thymidylate synthase from LCDV-C induces a transformed phenotype in fish cells. Virology, 372:118-126. 

[31] Zhao, Z., Ke, F., Huang, Y.H., Zhao, J. G., Gui, J.F., Zhang, Q.Y., 2008. Identification and characterization of a novel envelope protein in Rana grylio virus. J. Gen. Virol., 89: 1866 - 1872. 

[32] Zhao, Z., Ke, F., Li, Z,Q., Gui, J.F., Zhang, Q.Y., 2008. Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China. Arch Virol. 153: 1143-1148. 

[33] Tao, J.J., Zhou, G.Z., Gui, J.F., Zhang, Q.Y., 2008. Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF. Virus Res., 132: 86-96. 

[34] Li, Q., Kumar, A., Gui, J.F., Yu, F.S., 2008. Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2. Microb. Pathog. 44:426-434. 

[35] Du, X.Z., Zhou, L., Zhao, H. B., Wang, Y. F. and Gui, J.F., 2008. Identical sequences but different expression patterns of Hira gene in gynogenetic and gonochoristic crucian carps. Fish Physiol. Biochem., 34: 175-184. 

[36] Wang, D., Zhong, X.P., Qiao, Z.X., Gui, J.F., 2008. Inductive transcription and protective role of fish heme oxygenase-1 under hypoxic stress. The Journal of Experimental Biology, 211: 2700-2706. 

[37] Guo, W., Gui, J.F., 2008. Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio. Aquaculture International, 16: 497-510.  

[38] Liu, J.X., Zhai,Y.H., Geng, F.S., Xia, J.H., Gui, J.F., 2008. Molecular characterization and expression pattern of Fetuin-B in gibel carp (Carassiu auratus gibelio). Biochemical Genetics, 46: 620-633. 

[39] Xia, J.H., Liu, J.X., Zhou, L., Li Z., Gui, J.F., 2008. Apo-14 is required for digestive system organogenesis during fish embryogenesis and larval development. International Journal of Developmental Biology (Int. J. Dev. Biol.), 52: 1089-1098. 

[40] Wang,Y., Zhou,L., Li,Z., Gui, J.F.,2008.Molecular cloning and expression characterization of ApoC-I in orange-spotted grouper.Fish Physiology and Biochemistry, 34: 339-348.  

[41] Zhang, F.T., Zhang, Y.B., Chgen, Y.D., Zhu, R., Dong, C.W., Li, Y.Y., Zhang, Q.Y., Gui, J.F., 2008. Expressional induction of Paralichthys olivaceus Cathepsin B gene in response to virus, poly I:C and lipopolysaccharide. Fish and Shellfish Immunology, 25: 542-549.  


[42] Xia, W., Zhou,L., Yao,B., Li,C.J., Gui,J.F., 2007. Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers. Molecular and Cellular Endocrinology, 263: 156-172.  

[43] Zhang, Y.B., Jiang, Jun., Chen, Y.D., Zhu, R., Shi, Y., Zhang, Q.Y., Gui, J.F., 2007. The Innate Immune Response to Grass Carp Haemorrhagic Virus (GCHV) in Cultured Carassius auratus Blastulae (CAB) Cells. Developmental and Comparative Immunology, 31: 232-243.  

[44] Xu, H.Y., Li, M.Y., Gui,J.F., Hong,Y.H., 2007. Cloning and expression of medaka dazl during embryogenesis and gametogenesis. Mechanisms of Development-GEP, 7: 332-338.  

[45] Dong, C.W., Zhang, Y.B., Zhang, Q.Y. and Gui, J.F., 2007. Molecular characterization and inductive expression of a fish protein arginine methyltransferase 1 gene in response to virus infection. Fish and Shellfish Immunology, 22: 380-393.  

[46] Zhao, Z., Ke, F., Gui, J.F., Zhang, Q.Y., 2007. Characterization of an early gene encoding for dUTPase in Rana grylio virus. Virus Res., 123: 128-137.  

[47] Tao, J.J., Gui, J.F., Zhang, Q.Y., 2007. Isolation and characterization of a rhabdovirus from co-infection of two viruses in mandarin fish. Aquaculture, 262: 1-9.   

[48] Wang, H.Y., Zhou,L., Gui,J.F., 2007. Identification of a putative oocyte-specific small nuclear ribonucleoprotein polypeptide C in gibel carp. Comparative Biochemistry and Physiology Part B, 146: 47-52.  

[49] Zhu, H.P., Gui, J.F., 2007. Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio. Aquaculture, 265: 109-117.  

[50] Zhang, Y.B., Wang, Y.L., Gui, J.F., 2007. Identification and characterisation of two homologs of interferon stimulated gene ISG15 in crucian carp. Fish and Shellfish Immunology,23: 52-61.  

[51] Yao, B., Zhou, L., Wang, Y., Xia, W., Gui, J.F., 2007. Differential expression and dynamic changes of SOX3 during gametogenesis and sex reversal in protogynous hermaphroditic fish. J. Exp. Zool. 307A : 207-219.  

[52] Huang, Y.H., Huang, X.H., Zhang J,  Gui, J.F., ZhangQ.Y., 2007. Subcellular localization and characterization of chemokine receptor homolog from LCDV-C. Viral Immunology, 20: 150-159.  

[53] Zhang, Q.Y., Tao, J.J., Gui, L., Zhou, G. Z., Ruan, H.M., Li, Z.Q., Gui, J.F., 2007. Isolation and characterization of Scophthalmus maximusturbot rhabdovirus. Diseases of Aquatic Organisms, 74: 95-105. 

[54] Huang,Y.H., Huang,X.H., Gui J.F., Zhang,Q.Y., 2007.Mitochondria-mediated apoptosis induced by Rana glylio virus infection in fish cells. Apoptosis12: 1569-1577.  

[55] Zhou,L., Li,C.J., Wang,Y., Xia,W., Yao,B., Jin,J.Y., GuiJ.F., 2007. Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides). Journal of Chemical Neuroanatomy, 34: 47-59.  

[56] Yin, J., Xia, J.H., Du, X.Z., Liu, J., Zhou, L., Hong, Y.H., Gui, J.F., 2007. Developmental expression of CagMdkb during gibel carp embryogenesis. International Journal of Developmental Biology, 51: 761-769. 


[57] Zhu, H.P., Ma, D.M., Gui, J.F., 2006. Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting. Chromosome Research, 14 (2006): 767-776.  

[58] Chen, S.L., Wang, Z.J., Xu, M.Y., Gui, J.F., 2006. Molecular identification and expression analysis of natural resistance associated macrophage protein (Nramp) cDNA from Japanese flounder (Paralichthys olivaceus). Fish and Shellfish Immunology, 20: 365-373.  

[59] Zhang, Q.Y., Zhao, Z., Xiao, F., Li, Z.Q., Gui, J.F., 2006. Molecular characterization of three Rana grylio virus (RGV) isolates and Paralichthys olivaceus lymphocytes disease virus (LCDV-C) in iridoviruses. Aquaculture, 2511):1-10.  

[60] Liu, Y.M., Zhang, Q.Y., Yuan, X.P., Li, Z.Q., Gui, J.F., 2006. Seasonal variation of virioplankton in a eutrophic shallow lake. Hydrobiologia, 560: 323-334.  

[61] Ji, G.D., Zhou, L., Wang, Y., Xia, W., Gui, J.F., 2006. Identification of a novel C2 domain factor in ovaries of orange-spotted grouper (Epinephelus coioides). Comparative Biochemistry and Physiology Part B, 143: 374-383.  

[62] Dong, C.W., Zhang, Y.B., Zhang, Q.Y. and Gui, J.F., 2006. Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock. Fish and Shellfish Immunology, 21: 146-158.  

[63] Zhu, R., Zhang, Y.B., Chen, Y.D., Dong, C.W., Zhang, F.T., Zhang, Q.Y., Gui, J.F., 2006. Molecular cloning and stress-induced expression of paralichthys olivaceus heme-regulated initiation factor 2α kinase. Developmental and Comparative Immunology, 30: 1047-1059.  

[64] Zhou, L., Yao, B., Xia, W., Li, C.J., Wang, Y., Shi, Y.H., Gui, J.F., 2006. EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides) . Aquaculture, 256: 129-139.  

[65] Zhu, X.P., Wei, C.Q., Zhao, W.H., Du, H.J., Chen, Y.L., Gui, J.F., 2006. Effects of incubation temperatures on embryonic development in the Asian yellow pond turtle. Aquaculture, 259: 243-248. 

[66] Sun, W., Huang, Y., Zhao, Z., Gui, J.F., Zhang, Q. Y., 2006. Characterization of the Rana grylio virus 3beta-hydroxysteroid dehydrogenase and its novel role in suppressing virus-induced cytopathic effect. Biochem Biophys Res Commun. 351: 44-50.  

Monographs and Textbooks:  

[1] Zhang, Q.Y., Gui, J.F., Aquatic Virology, Higher Education Press, Beijing, 2008, pp1-414. 

[2] Gui, J.F. et al., Genetic Basis and Artificial Control of Sexuality and Reproduction in Fish. Science Press, Beijing, 2007. pp 1-247. 

[3] Gui, J.F., Shi, Y.D., Zhou, L., Aquaculture Techniques of Allogynogenetic Gibel Carp. Jin-Dun Press, Beijing, 2003, pp 1-138. 

[4] Gui, J.F., Yi, M.S. et al., Developmental Biology. Science Press, Beijing, 2002. pp 1-563. 

[5] Wu, C.J., Gui, J.F. et al., Fish Genetic Breeding Engineering, Shanghai Science and Technology Press, 1999, pp 1-286. 

[6] Gui, J.F., RNA Processing and Cell Cycle Regulation. Science Press, Beijing, 1998. pp 1-227.