Research Group of Fish Cytogenetics and Population Genetics
PI: Prof. TONG Jingou, PhD
Members: Xiaomu Yu, and 7 postgraduate candidates
Prof. J. Tong,
7 East Lake Road South,
Institute of Hydrobiology, the Chinese Academy of Sciences,
Wuhan 430072, China.
Tel: +86 27 6878 0751
Fax: +86 27 6878 0123.
The Research Group of Fish Cytogenetics and Population Genetics mainly focuses on genetic analyses of economic traits and basic and applied basic studies on genetics and breeding in major aquaculture fish.
Over the past decade, this group has been financially supported by eight national, provincial or ministrial research funds such as the National Key Basic Research Development Program, National Key Research and Development Program, National Science Foundation, Special Fund for Nonprofit (Agricultural) Industry Research Program, and Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences. They also published around 50 key journal articles, and three granted patents of invention. More than 10 M.Sc and Ph.D students were graduated from this group up to now.
Current research interests:
* Construction of pure lines in fish via cytogenetic/cytoengineering methodologies
* By means of genome-wide association study and other omics and molecular genetic approaches, this group tried to reveal genetic mechanisms for growth, body shape and color, sex determination, stress resistance, and identify genes and their regulatory elements potentially useful in breeding programs
* Applying marker-assisted selection, molecular design breeding etc., this group tried to design varieties (strains) and speed domestication, selection and genetic improvement mainly in bighead carp (Hypophthalmichthys nobilis), in order to provide scientific supports from seed industry for ecological/water-conservation fisheries in China. In this aspect, a strain of bighead carp with good quality of growth and body shape is under culture testing.
Tong J.*, Wang D., Cheng L. 2009. Development of microsatellite markers by data mining from DNA sequences. In: Data mining and knowledge discovery in real life applications. I-Tech Education and Publishing KG, Vienna, Austria.
You C., Yu X., and Tong J.* 2009. Detection of hybridization between two loach species (Paramisgurnus dabryanus and Misgurnus anguillicaudatus) in wild populations. Environmental Biology of Fishes, Online available (DOI 10.1007/s10641-007-9282-x)
Wang D., Tan D., Tong J.* 2009. Extraction and amplification of genomic DNA from formalin-fixed Coreius heterodon. Chinese Journal of Ecology, 28: 572-576.
Chan T.-Y., Tong J., Tam Y.K.,Chu K.H. 2008. Phylogenetic relationships among genera of the Penaeidae (Crustacea: Decapoda) revealed by mitochondrial 16S rRNA gene sequences. Zootaxa, 1694: 38-50.
Cheng L, Liu L, Yu X, Tong J.* 2008. Sixteen polymorphic microsatellites in bighead carp (Aristichthys nobilis), and cross-amplification in silver carp. Molecular Ecology Resources, 8: 656-658.
You C., Yu X., Tan D.Q., Tong J.* 2008. Gynogenesis and sex determination in large scale loach, Paramisgurnus dabryanus (Sauvage). Aquaculture International, 16: 203-214.
Zheng J.S., Liao X.L., Tong J.G., Du H.J., Milinkovitch M.C., Wang D. 2008. Development and characterization of polymorphic microsatellite loci in endangered Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis). Conservation Genetics, 9: 1007-1009.
Wang C.Y., Yu X., Tong J.* 2008. Development and characterization of polymorphic EST markers in common carp. Acta Hydrobiologia Sinica, 32: 207-212.
Zheng J.S., Liao X., Tong J., Wang D. 2008. A pilot study on isolation of microsatellite DNA in the Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis). Acta Hydrobiologia Sinica, 32: 19-25.
Cheng L, Yu X, Liao X, Tong J*. 2007. Development of EST-SSRs by anefficient FIASCO-based strategy: a case studyin rare minnow (Gobiocypris rarus). Animal Biotechnology, 18:1-10.
Li WT, Liao XL, Yu XM, Cheng L, Tong J*. 2007. Isolation and characterization of polymorphic microsatellites in a sex reversal fish, rice field eel (Monopterus albus). Molecular Ecology Notes, 7: 705-707.
Li WT, Liao XL, Yu XM, Wang D, and Tong J*. 2007. Isolation and characterization of polymorphic microsatellite loci in Wuchang bream (Megalobrama amblycephala). Molecular Ecology Notes, 7: 771-773.
Liao X, Wang D, Yu X, Li W, Cheng L, Wang J, Tong J.* 2007. Characterization of novel microsatellite loci in rare minnow (Gobiocypris rarus) and amplification in closely-related species in Gobioninae. Conservation Genetics, 8: 1003-1007.
Liao X, Yu X, Chang J, Tong J.* 2007. Polymorphic microsatellites in largemouth bronze gudgeon (Coreius guichenoti) developed from repeat-enriched libraries and cross-species amplifications. Molecular Ecology Notes, 7: 1104-1107.
Wang C, Yu X, Tong J.* 2007. Microsatellite diversity and population genetic structure of redfin culter (Culter erythropterus) in fragmented lakes of the Yangtze River. Hydrobiologia. 586: 321–329.
Wang D, Liao XL, Cheng L, Yu XM, Tong J.* 2007.Development of novel EST-SSR markers from common carp by data mining from public EST sequences. Aquaculture. 271: 558-574.
Liao X., Zhu B., Yu X., Tan D., Chang J., Tong J.* 2006. Isolation and characterization of polymorphic microsatellites in a Yangtze River fish, brass gudgeon (Coreius heterodon Bleeker). Molecular Ecology Notes, 6: 393-395.
Liao X.L., Yu X.M., Tong J.* 2006. Genetic diversity of common carp from two largest Chinese lakes and the Yangtze River revealed by microsatellite markers. Hydrobiologia, 568: 445-453.
Maneeruttanarungroj C., Pongsomboon S., Wuthisuthimethavee S., Klinbunga S., Wilson K.J., Swan J., Li Y., Whan V., Chu K.H., Li C.P., Tong J., Glenn K., Rothschild M., Jerry D., Tassanakajon A. 2006. Development of polymorphic expressed sequence tag-derived microsatellites for the extension of the genetic linkage map of the black tiger shrimp (Penaeus monodon). Animal Genetics, 37: 363-368.
Wang J.X., Liu H.J., Min W.Q., Tong J., Guan M., Han Y.Z., Gong L.J., Huang Z., Ren J., Zhang J.P., and Zheng H.P. 2006. Induced meiotic gynogenesis in tench, Tinca tinca (L.) using irradiated heterogenic sperm. Aquaculture International, 14: 35-40.
Zhang B., Li Z.J., Tong J.G, Liao X.L. 2006. Isolation and characterization of 18 polymorphic microsatellite markers in Chinese mandarin fish Siniperca chuatsi (Basilewsky). Molecular Ecology Notes, 6: 1216-1218.
Zhang X., Tong J., and Xiong B. 2006. Applications of microsatellite markers on studies of genetics and breeding of fishes. Chinese Journal of Agricultural Biotechnology, 3: 83-87.
Zhong L., Yu X., Tong J.* 2006. Sox genes in grass carp (Ctenopharyngodon idella) with their implications for genome duplication and evolution. Genetics Selection and Evolution, 38: 673-687.
Gen B., Sun X.W., Liang L.Q., Ouyang H.S., Tong J. 2006. Analyses of genetic diversity of bighead carp using 17 microsatellites. Hereditas (Beijing), 28: 683-688.
Tong J.*, Yu X., Liao X., 2005. Characterization of a highly conserved microsatellite marker with utility potentials in cyprinid fishes. Journal of Applied Ichthyology, 21: 232-235.
Liao X., Yu X., Tan D., Tong J.* 2005. Microsatellite diversity of grass carp from the Yangtze River basin. Acta Hydrobiologia Sinica, 29: 113-119.