Center for Molecular and Cellular Biology of Aquatic Organisms

Research Group of Protozoan Functional Genomics




PI: Prof. MIAO Wei, PhD 

Members: Jie XIONG, Dongxia YUAN;Graduate students: Yue CHANG, Miao TIAN, Qiuping ZHONG, Guangying WANG, Jing ZHANG  

Contact Us: Institute of Hydrobiology(IHB), Chinese Academy of Sciences(CAS), 7 Donghu South Road, Wuhan, 430072, Hubei, P.R.China;      

Tel/Fax: +86 (0)27-68780050  

General Introduction:  

The Research Group of Protozoan Functional Genomics succeeds the well-reputed Research Group of Protozoan Taxonomy and Ecology which was founded by Prof. WANG Jiaji (CAS member) and further developed by Prof. SHEN Yunfen (CAS member).  

Focusing on the protists especially the model system Tetrahymena, this group is interested in understanding the unique mechanisms of molecular evolution, functional divergence and ecological adaptation in the unicellular eukaryotes at the genomics level, and the basic metabolism pathways or regulation networks that are conserved in eukaryotes.  

This group established and maintained two important database resources of Tetrahymena, including the Tetrahymena Functional Genomics Database (TetraFGD, and Tetrahymena Comparative Genomics Database (TCGD, It also launched the Protist 10000 Genomes Project (P10K). 

Main research directions:  

* Biosystematics of Protozoa 

* Evolutionary and Ecological Functional Genomics of Protozoa 

* Molecular toxicology of Protozoa; 4) Synthetic biology techniques of Protozoa.  

Recent Publications:    

(1)Cervantes MD, Hamilton EP, Xiong J, Lawson MJ, Yuan D, Hadjithomas M, Miao W*, Orias E*. 2013. Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila [J]. Plos Biology, 11(3): e1001518. 

(2)Xiong J, Lu YM, Feng JM, Yuan DX, Tian M, Chang Y, Fu CJ, Wang GY, Zeng HH*, Miao W*, Tetrahymena Functional Genomics Database (TetraFGD): an integrated resource for Tetrahymena functional genomics. Database. 2013 : bat008 

(3)Xiong J, Lu XY, Zhou ZM, Chang Y, Yuan DX, Tian M, Zhou ZG, Wang L, Fu CJ, Orias E, Miao W*. 2012. Transcriptome Analysis of the Model Protozoan, Tetrahymena Thermophila, Using Rna-Seq [J]. Plos One, 7(2): e30630. 

(4)Xiong J, Yuan D, Fillingham JS, Garg J, Lu X, Chang Y, Liu Y, Fu C, Pearlman RE, Miao W*. 2011. Gene Network Landscape of the Ciliate Tetrahymena Thermophila [J]. Plos One, 6(5): e20124. 

(5)Chang Y, Feng LF, Miao W*. 2011. Toxicogenomic Investigation of Tetrahymena Thermophila Exposed to Dichlorodiphenyltrichloroethane (DDT), Tributyltin (TBT), and 2,3,7,8-Tetrachlorodibenzo-P-Dioxin (TCDD) [J]. Sci China Life Sci, 54(7): 617-625. 

(6)Xiong J, Feng L, Yuan D, Fu C, Miao W*. 2010. Genome-Wide Identification and Evolution of Atp-Binding Cassette Transporters in the Ciliate Tetrahymena Thermophila: A Case of Functional Divergence in a Multigene Family [J]. Bmc Evol Biol, 10: 330. 

(7)Fu C, Xiong J, Miao W*. 2009. Genome-Wide Identification and Characterization of Cytochrome P450 Monooxygenase Genes in the Ciliate Tetrahymena Thermophila [J]. BMC Genomics, 10:208. 

(8)Miao W*, Xiong J, Bowen J, Wang W, Liu Y, Braguinets O, Grigull J, Pearlman RE, Orias E, Gorovsky MA*. 2009. Microarray Analyses of Gene Expression During the Tetrahymena Thermophila Life Cycle [J]. Plos One, 4(2): e4429. 

(9)Guo L, Fu C, Miao W*. 2008. Cloning, Characterization, and Gene Expression Analysis of a Novel Cadmium Metallothionein Gene in Tetrahymena Pigmentosa [J]. Gene, 423(1): 29-35. 

(10)Feng LF, Miao W*, Wu YX. 2007. Difterentially Expressed Genes of Tetrahymena Thermophila in Response to Tributyltin (TBT) Identified by Suppression Subtractive Hybridization and Real Time Quantitative Pcr [J]. Aquatic Toxicology, 81(1): 99-105. 

(11)Fu CJ, Miao W*. 2006. Cloning and Characterization of a New Multi-Stress Inducible Metallothionein Gene in Tetrahymena Pyriformis [J]. Protist, 157(2): 193-203. 

(12)Miao W*, Yu T, Orias E, Wan ML, Fu CJ. 2006. Identification of Differentially Expressed Genes in Tetrahmena Thermophila in Response to Dichlorodiphenyltrichloroethane (DDT) by Suppression Subtractive Hybridization [J]. Environmental Microbiology, 8(6): 1122-1129. 

(13)Miao W, Simpson AGB, Fu CJ, Lobban CS. 2005. The Giant Zooxanthellae-Bearing Ciliate Maristentor Dinoferus (Heterotrichea) Is Closely Related to Folliculinidae [J]. J Eukaryot Microbiol, 52(1): 11-16. 

(14)Miao W, Fen WS, Yu YH, Zhang XY, Shen YF. 2004. Phylogenetic Relationships of the Subclass Peritrichia (Oligohymenophorea, Ciliophora) Inferred from Small Subunit rRNA Gene Sequences [J]. J Eukaryot Microbiol, 51(2): 180-186. 

(15)Miao W, Yu YH, Shen YF, Zhang XY. 2004. Intraspecific Phylogeography of Carchesium Polypinum (Peritrichia, Ciliophora) from China, Inferred from 18s-ITS1-5.8s Ribosomal DNA [J]. Sci China Ser C, 47(1): 11-17. 

*: corresponding Author