Center for Molecular and Cellular Biology of Aquatic Organisms

Research Group of Fish Hypoxia Biology




PI: Prof. XIAO Wuhan, PhD

Members: Xing Liu, Ph.D., Associate Professor; Jing Wang, Ph.D., Associate Professor; Gang Ouyang, M.S. Lab Manager.

Contact Us: 86-27-68780087 (office), 86-27-68780092 (lab)

General Introduction:  The Research Group of Fish Hypoxia Biology mainly uses economic fish and model fish (Zebrafish) as research materials.  

By gene editing, gene regulation, protein/protein interaction and gene function analysis (in vitro and in vivo) , they aim to investigate fish in hypoxia tolerance and adaptation, innate immunity in response to viral infection and try to demonstrate the molecular mechanisms of the crosstalk between hypoxia signaling pathway and antiviral innate immunity signaling pathway.  

They seek to establish a foundation for molecular designing new fish strains with hypoxia tolerance and antiviral infection.  

Current research interests:  

Molecular mechanisms of fish in hypoxia tolerance and the genetic basis in breeding new fish strains with hypoxia tolerance 

*  Molecular mechanisms of fish in antiviral infection and the genetic basis in breeding new fish strains with antiviral infection 

*  Molecular mechanisms of the crosstalk between hypoxia signaling pathway and antiviral innate immunity    

Selected Publications (*corresponding author) : 


(1) Sijia Fan , Jing Wang, Guangqing Yu , Fangjing Rong, Dawei Zhang, Chenxi Xu , Juan Du, Zhi Li , Gang Ouyang , Wuhan Xiao* . TET is targeted for proteasomal degradation by the PHD-pVHL pathway to reduce DNA hydroxymethylation. Journal of Biological Chemistry, 2020, doi: 10.1074/jbc.RA120.014538. (

2) Zhi Li , Sijia Fan , Jing Wang , Xiaoyun Chen, Qian Liao , Xing Liu , Gang Ouyang, Hong Cao , Wuhan Xiao* . Zebrafish F-box protein fbxo3 negatively regulates antiviral response through promoting K27-linked polyubiquitination of the transcription factors irf3 and irf7. Journal of Immunology, 2020, 205(7):1897-1908.

(3) Xiaolian Cai , Ziwen Zhou, Junji Zhu , Qian Liao , Dawei Zhang , Xing Liu , Jing Wang , Gang Ouyang , Wuhan Xiao* . Zebrafish hif-3α modulates erythropoiesis via regulation of gata-1 to facilitate hypoxia tolerance. Development, 2020, doi: 10.1242/dev.185116.

(4) Guangqing Yu , Xing Liu , Dawei Zhang, Jing Wang, Gang Ouyang , Zhu Chen, Wuhan Xiao *. Zebrafish nedd8 facilitates ovarian development and the maintenance of female secondary sexual characteristics via suppression of androgen receptor activity. Development, 2020, 147(18): dev.194886.

(5) Guangqing Yu , Xiong Li , Ziwen Zhou , Jinhua Tang , Jing Wang, Xing Liu, Sijia Fan , Gang Ouyang, Wuhan Xiao * . Zebrafish phd3 negatively regulates antiviral responses via suppression of irf7 transactivity independent of its prolyl hydroxylase activity. Journal of Immunology, 2020, 205(4):1135-1146.

(6) Gang Ouyang , Qian Liao , Dawei Zhang , Fangjing Rong , Xiaolian Cai, Sijia Fan , Junji Zhu , Jing Wang , Xing Liu , Xueqin Liu, Wuhan Xiao * . Zebrafish NF-κB/p65 is required for antiviral responses. Journal of Immunology, 2020, 204(11):3019-3029.

(7) Xing Liu , Chunchun Zhu, Huangyuan Zha , Jinhua Tang , Fangjing Rong, Xiaoyun Chen , Sijia Fan , Chenxi Xu , Juan Du, Junji Zhu, Jing Wang , Gang Ouyang , Guangqing Yu , Xiaolian Cai , Zhu Chen , Wuhan Xiao *. SIRT5 impairs aggregation and activation of the signaling adaptor MAVS through catalyzing lysine desuccinylation. EMBO Journal, 2020, 39(11): e103285.

(8) Junji Zhu, Xing Liu, Xiaolian Cai, Gang Ouyang, Sijia Fan, Jing Wang, Wuhan Xiao*. Zebrafish prmt7 negatively regulates antiviral response by suppressing the retinoic acid-inducible gene 1-like receptor signaling. FASEB Journal, 2020, 34(1): 988-1000.

(9) Junji Zhu , Xing Liu , Xiaolian Cai , Gang Ouyang , Huangyuan Zha , Ziwen Zhou , Qian Liao , Jing Wang , Wuhan Xiao*. Zebrafish prmt3 negatively regulates antiviral responses. FASEB Journal, 2020, doi: 10.1096/ fj.201902569R


(10) Junji Zhu, Dawei Zhang, Xing Liu, Guangqing Yu, Xiaolian Cai, Chenxi Xu, Fangjing Rong, Gang Ouyang, Jing Wang, Wuhan Xiao*. Zebrafish prmt5 arginine methyltransferase is essential for germ cell development. Development, 2019, 146(20): dev179572.

(11) Guangqing Yu, Xing Liu, Jinghua Tang, Chenxi Xu, Gang Ouyang, Wuhan Xiao*. Neddylation Facilitates the Antiviral Response in Zebrafish. Frontiers in Immunology, 2019, 10: 1432.


(12) Xiaolian Cai, Dawei Zhang, Jing Wang, Xing Liu, Gang Ouyang, Wuhan Xiao*. Deletion of the fih gene encoding an inhibitor of hypoxia-inducible factors increases hypoxia tolerance in zebrafish. Journal of Biological Chemistry, 2018, 293(40): 15370-15380.

(13) Chenxi Xu, Xing Liu, Huangyuan Zha, Sijia Fan, Dawei Zhang, Wuhan Xiao*. A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1α protein. PLoS Pathogens, 2018, 18(8): e1007259.


(14) Jing Wang, Dawei Zhang, Juan Du, Chi Zhou, Zhi Li, Xing Liu, Gang Ouyang, Wuhan Xiao*. Tet1 facilitates hypoxia tolerance by stabilizing the HIF-α proteins independent of its methylcytosine dioxygenase activity. Nucleic Acids Research, 2017,45(22): 12700-12714.

(15) Dawei Zhang, Jing Wang, Chi Zhou, Wuhan Xiao*. Zebrafish akt2 is essential for survival, growth, bone development, and glucose homeostasis. Mechanisms of Development, 2017, 143:42-52.


(16) Xing Liu, Xiaolian Cai, Dawei Zhang, Chenxi Xu, Wuhan Xiao*. Zebrafish foxo3b negatively regulates anti-viral response through suppressing the transactivity of irf3 and irf7. Journal of Immunology, 2016, 197(12): 4736-4749.

(17) Xing Liu, Xiaolian Cai, Bo Hu, Zhichao Mei, Dawei Zhang, Gang Ouyang, Jing Wang, Wei Zhang, Wuhan Xiao*. Forkhead transcription factor (FOXO3a) modulates hypoxia signaling via up-regulation of the von Hippel-Lindau gene (VHL). Journal of Biological Chemistry, 2016, 291(49): 25692-25705.

(18) Yu Chen, Chi Zhou, Wei Ji, Zhichao Mei, Bo Hu, Wei Zhang, Dawei Zhang, Jing Wang, Xing Liu, Gang Ouyang, Jiangang Zhou, Wuhan Xiao*. ELL targets c-Myc for proteasomal degradation and suppresses tumor growth. Nature Communications, 2016, 7: 11057.


(19) Xing Liu, Zhu Chen, Gang Ouyang, Tieshan Song, Huageng Liang, Wei Liu, Wuhan Xiao*. ELL associated factor 2 (EAF2) inhibits transforming growth factor beta signaling through a direct interaction with Smad3. Journal of Biological Chemistry, 2015, 290(43): 25933-25945.

(20) Juan Du, Dawei Zhang. Wei Zhang, Gang Ouyang, Jing Wang, Xing Liu, Sun Li, Wei Liu, Wuhan Xiao*. pVHL negatively regulates antiviral signaling by targeting MAVS for proteasomal degradation. Journal of Immunology, 2015, 195(4): 1782-1790.

(21) Zhichao Mei, Dawei Zhang, Bo Hu, Jing Wang, Xian Shen, Wuhan Xiao*. FBXO32 targets c-Myc for proteasomal degradation and inhibits c-Myc activity. Journal of Biological Chemistry, 2015, 290(26): 16202-16214.

(22) Xing Liu, Zhu Chen, Chenxi Xu, Xiaoqian Leng, Hong Cao, Gang Ouyang, Wuhan Xiao*. Repression of hypoxia-inducible factor alpha signaling by Set7-mediated methylation. Nucleic Acids Research, 2015, 43(10): 5081-5098.


(23) Bo Hu, Wei Zhang, Xi Feng, Wei Ji, Xunwei Xie, Wuhan Xiao*. Zebrafish eaf1 suppresses foxo3b expression to modulate transcriptional activity of gata1 and spi1 in primitive hematopoiesis, Developmental Biology, 2014, 388: 81-93.

(24) Jing Wang, Wei Zhang, Wei Ji, Xing Liu, Gang Ouyang, Wuhan Xiao*. The Von Hippel-Lindau protein suppresses androgen receptor activity. Molecular Endocrinology, 2014, 28(2): 239-248.

(25) Zhu Chen, Xing Liu, Zhichao Mei, Zhou Wang*, Wuhan Xiao*. EAF2 suppresses HIF-1a transcriptional activity by disrupting its interaction with the co-activator CBP/p300. Molecular and Cellular Biology, 2014, 34(6): 1085-1099.

(26) Wei Zhang, Wei Ji, Xing Liu, Gang Ouyang, Wuhan Xiao*. ELL inhibits E2F1 transcriptional activity by enhancing E2F1 deacetylation via recruitment of Histone Deacetylase 1. Molecular and Cellular Biology, 2014, 34(4): 765-775.

(27) Wei Ji, Jing Wang, Wei Zhang, Xing Liu, Gang Ouyang, Wuhan Xiao*. pVHL acts as a downstream target of E2F1 to suppress E2F1 activity. Biochemical Journal, 2014, 457: 185-195.


(28) Jing-xia Liu, Dawei Zhang, Xunwei Xie, Gang Ouyang, Xing Liu, Yonghua Sun, Wuhan Xiao*. Eaf1 and Eaf2 negatively regulate canonical Wnt/beta-catenin signaling. Development, 2013, 140: 1067-1078.


(29) Xiaoyang Wan, Bo Hu, Jing-xia Liu, Xi Feng, Wuhan Xiao*. Zebrafish mll gene is essential for hematopoiesis. Journal of Biological Chemistry, 2011, 286: 33345-33357

(30) Xi Feng, Xing Liu, Wei Zhang, Wuhan Xiao*. p53 directly suppresses BNIP3 expression to protect against hypoxia-induced cell death. EMBO Journal, 2011, 30: 3397-3415.


(31) Jiangang Zhou, Xi Feng, Bing Ban, Jing-xia Liu, Zhou Wang*, Wuhan Xiao*. The elongation factor ELL (Eleven-Nineteen Lysine-Rich Leukemia) acts as a transcription factor for direct thrombospondin-1 regulation. Journal of Biological Chemistry, 2009, 284(28):19142-19152.

(32) Jing-xia Liu, Bo Hu, Yang Wang, Jianfang Gui, Wuhan Xiao*. Zebrafish eaf1 and eaf2/u19 mediate effective convergence and extension movements through the maintenance of wnt11 and wnt5 expression. Journal of Biological Chemistry, 2009, 284(24):16679-16692.