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  • Research Group of Cell Biology of Green Algae and Cilia
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     Prof. HUANG Kaiyao
    Principal Investigator:
    Prof
    . HUANG Kaiyao

    Members: Dr. Huan Long, Xuan Deng, Rongli Miao, Hui Zeng, Zedong Xu, Kun Wang, Jun Zhang, Huifang Sun

    Contact Us:

    Address: 7 South Donghu Road, Wuhan, 430072, China

    Telephone: 0086-27-68780902

    General Introduction:

    Modern cell biology has made great advances in our understanding of the structure and function of organelles. However, detailed knowledge of biogenesis (assembly) and turnover (disassembly) of organelles is lacking. We are using the flagella/cilia and oil bodies as models to address how hundreds of proteins and lipids assemble into organelles, and how organelles disassemble temporally and spatially.

    Cilia and flagella are hair-like organelles emanating from the cell surface and are excellent models to study the biogenesis and turnover of organelles. Their assembly and disassembly are coordinated with the cell cycle and can be induced experimentally. The length of cilia and flagella corresponds to the size of the organelle and can be used to quantify the rate of assembly and disassembly. The primary model of ciliary/flagellar research is the eukaryotic unicellular alga Chlamydomonas, whose flagella share the same structure and essential proteins with cilia in human. It is suited for biochemical and genetic studies since it can be manipulated like yeast. Our work combines biochemical, proteomic, genetic, cell biological and live imaging methods to study flagellar membrane biogenesis and the disassembly of cilia/flagella.

    Oil bodies, also called lipid droplets in animals and lipid particles in yeast, are intracellular storage of neutral lipids surrounded by a monolayer of phospholipid with attached or embedded proteins. They are main resources for making renewable energy biodiesel. The recent bloom of researches on the lipid droplets formation in animals has provided many new insights of this organelle, but the correlation between the photosynthesis and formation of the oil bodies only can be addressed in plants. Green algae such as Botryococcus accumulate oil up to 70% in their dry weight and are suitable models for studying the formation and secretion of oil bodies.

     
     Chief Members

    Recent Publications:

    1.    Huang K., Tsao C., 2010, Importin-β2: a key to two gates? 2010, Protein&Cell 1(9):791-792. (Huang K is corresponding author)

    2.    Huang K., Diener D., Rosenbaum J., 2009, The ubiquitin conjugation system is involved in the disassembly of cilia/flagella. Journal of Cell Biology, 186:601-613.

    3.    Huang K., Diener D., Mitchell A., Pazour G., Witman G., Rosenbaum J., 2007, Function and dynamics of PKD2 in Chlamydomonas flagella. Journal of Cell Biology, 179:501-514.

    4.    Soon Im C., Eberhard S., Huang  K., Beck C., Grossman A., 2006. Phototropin involvement in expression of genes encoding chlorophyll and carotenoid biosynthesis enzymes and LHC apoproteins in Chlamydomonas reinhardtii. Plant Journal 48(1):1-16.

    5.    Huang K., Kunkel T., Beck. C. F., 2004. Localization of blue-light receptor phototropin to the flagella of the green alga Chlamydomonas reinhardtii. Mol. Biol. Cell. 15(8):3605-3614.

    6.    Huang K., Beck C.F., 2003. Phototropin is the blue-light receptor that controls multiple steps in the sexual life cycle of the green alga Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA. 100:6269-6274.

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    Address: No. 7 Donghu South Road, Wuchang District, Wuhan, Hubei Province