Research Group of Aquatic Virology
PI: Associate Prof. KE Fei, PhD
ZHANG Qiya (PhD / Professor),
Fei KE (PhD/Associate Professor)
Li-Ming XU (PhD/Associate Professor/Postdoctor)
Graduate student: Xiao-Tao ZENG, Nai-Tong YU, Yu-Han ZHAO, Zi-Hao Wang, Cheng-Yue Ming
The Research Group of Aquatic Virology has been devoted to the study of aquatic animal viruses that cause epidemic diseases and the aquatic microbial viruses that drive the earth's biogeochemical cycles at the nanometer scale. It also studies the molecular biology of these viruses.
Over the past 24 years since the establishment of this group, the group members, led by Prof. ZHANG Qiya, have been working together on the isolation of aquatic virus and elucidated their genome architectures and key gene functions. They also investigated virus replication cycles, molecular genetics and evolution, molecular mechanisms of virus-host and virus-aquatic environment; developed aquatic virus detection and prevention technology; sought for ways of utilizing aquatic virus resources.
Based on the established research technical system of aquatic virology, this group has isolated different viral pathogens from diseased aquatic animals including amphibian, fish, and crustacea, and from harmful algae; resolved whole genome of 13 aquatic viruses from six families; illustrated the organization characters and biological functions of a batch of key genes from aquatic virus; and revealed the molecular mechanism of ranavirus pathogenesis and cross-species infection.
Their related achievements won two Natural Science Prizes of Hubei Province. Eight invention patents were authorized. The bilingual monograph Atlas of Aquatic Viruses and Viral Diseases, together with the graduate textbook Aquatic Virology and a large number of research papers, were published and widely cited by experts at home and abroad.
It is a capable and highly effective team, providing the source of strength for the growth of young talents. More than 40 graduate students have been trained, many of whom won awards like the Excellent President Award of the CAS, Excellent Doctoral Dissertation Award of Hubei Province, and National Scholarship for Graduate Students. More than 60% of the graduate students have obtained the title of associate professor or above, or have management responsibilities at the same time. They play important role in the corresponding fields at home and abroad.
In the future, this group will continue to focus on the aquatic virus and its molecular biology research, especially on the mechanisms of entry and genome replication and transcription of aquatic viruses; investigate how aquatic viruses invade and infect in different host; interpret the mechanism of aquatic virus infection and cross-species transmission; and look for technologies of resistance to virus disease or utilization and modification of virus as biological resources.
Major Areas of Research:
1. Isolation and identification of emerging viral pathogens, characterization of aquatic virus genome structure and gene function.
2. Elucidation of aquatic virus life cycle, pathogenesis and aquatic virus-host interaction, and the interaction of viruses and their hosts in aquatic environment.
3. Development of the strategies for preventing, diagnosing and controlling viral diseases in aquaculture, and exploration of aquatic virus resources.
Molecular mechanisms of the pathogenesis and transmission of aquatic animal viruses; mechanisms of aquatic animal virus genome replication and transcription; aquaculture animal virus-host interactions and host antiviral mechanisms.
1. Genome anatomy of different aquatic animal viruses and molecular mechanism of virus-host interactions, Second grade prize of natural science of Hubei province, 2017.
2. Identification of important viral pathogens from aquaculture animals and mechanisms of their pathogenesis, Second grade prize of natural science of Hubei province, 2004.
Ke F, Gui JF, Chen ZY, Li T, Lei CK, Wang ZH, Zhang QY. 2018. Divergent transcriptomic responses underlying the ranaviruses-amphibian interaction processes on interspecies infection of Chinese giant salamander. BMC Genomics. 19(1):211.
Chen ZY, Li T, Gao XC, Wang CF, Zhang QY. 2018. Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus. Viruses. 10(2). pii: E52.
Cao YS, Wang D, Li SW, Xu LM, Zhao JZ, Liu HB, Lu TY, Zhang QY. 2018. Identification and analysis of differentially expressed microRNAs in rainbow trout (Oncorhynchus mykiss) responding to infectious hematopoietic necrosis virus infection. Developmental and Comparative Immunology, 88(2018):28-36
Gui L, Chinchar VG, Zhang QY. 2018. Molecular basis of pathogenesis of emerging viruses infecting aquatic animals. Aquaculture and Fisheries. 3(1)1-5
Gui L, Li T, Zhang QY. 2018. Fish kidney cells show higher tolerance to hyperosmolality than amphibian. Aquaculture and Fisheries. 3(3): 135-138
Chinchar VG, Hick P, Ince IA, Jancovich JK, Marschang R, Qin Q, Subramaniam K, Waltzek TB, Whittington R, Williams T, Zhang QY, Ictv Report Consortium. 2017. ICTV Virus Taxonomy Profile: Iridoviridae. Journal of General Virology. 98(5):890-891.
Zeng XT, Chen ZY, Deng YS, Gui JF, Zhang QY. 2016. A novel fish herpesvirus genome without terminal direct repeat and its molecular diagnosis. Archives of Virology. 161(12):3577-3581.
Liu J, Pei C, Gao X, Chen ZY, Zhang QY. 2016. Fish reovirus GCRev-109 VP33 protein elicits protective immunity in rare minnows. Archives of Virology. 161(3):573-82
Cao YS, Xu LM. LaPatra SE, Zhao JZ, Liu M, Liu HB, Lu TY, Zhang QY. 2016. The kinetics and protection of the antiviral state induced by recombinant iIFN1a in rainbow trout against infectious hematopoietic necrosis virus. Molecular Immunology. 76:55-61
Wang J, Gui L, Chen ZY, Zhang QY. 2016. Mutations in the C-terminal region affect subcellular localization of crucian carp herpesvirus (CaHV) GPCR. Virus Genes. 52:484-494
Gao XC, Chen ZY, Liu J, Zhang QY. 2016. Development and application of monoclonal antibodies for detection and analysis of aquareoviruses. Journal of Immunoassay and Immunochemistry. 37(4): 376-389
Huang X, Fang J, Chen ZY, Zhang, QY. 2016. Rana grylio virus TK and DUT gene locus could be simultaneously used for foreign gene expression. Virus Research, 214: 33-38
Zhang QY, Gui JF. 2015. Virus genomes and virus-host interactions in aquaculture animals. Sci China Life Sci. 58(2): 156–169
Gao XC, Chen ZY, Yuan JD, Zhang QY. 2015. Morphological changes in amphibian and fish cell lines infected with Andrias davidianus ranavirus. Journal of Comparative Pathology. 152(2-3):110-113
Ou T, Liao XY, Gao XC, Xu XD, Zhang QY. 2015. Unraveling the genome structure of cyanobacterial podovirus A-4L with long direct terminal repeats. Virus Research. 203:4-9
Yuan JD, Chen ZY, Huang X, Gao XC, Zhang QY. 2015. Establishment of three cell lines from Chinese giant salamander and their sensitivities to the wild-type and recombinant ranavirus. Veterinary Research. 46:58
Chen ZY, Gao XC, Zhang QY. 2015. Whole-genome analysis of a novel fish reovirus (MsReV) discloses aquareovirus genomic structure relationship with host in saline environments. Viruses. 7(8): 4282-4302
Ou T, Gao XC, Li SH, Zhang Q. 2015. Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host. Journal of General Virology. 96:3681-3697
He LB, Ke F, Wang J, Gao XC, Zhang QY. 2014. Rana grylio virus (RGV) envelope protein 2L subcellular localization and essential roles in virus infectivity revealed by conditional lethal mutant. Journal of General Virology, 95: 679-690
Zhu RL, Zhang QY. 2014. Determination and analysis of the complete genome sequence of Paralichthys olivaceus rhabdovirus (PORV). Archives of Virology. 159: 817-820
Pei C, Ke F, Chen ZY, Zhang QY. 2014. Complete genome sequence and comparative analysis of grass carp reovirus strain 109 (GCReV-109) with other grass carp reovirus strains reveals no significant correlate regional distribution. Archives of Virology. 159: 2435-2440
Ou T, Lei XY, He LB, Zhou FJ, Zhang QY. 2014. Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses. Virus Research. 189: 56-62
Zhu R, Chen ZY, Wang J, Yuan JD, Liao XY, Gui JF, Zhang QY Extensive diversification of MHC in Chinese giant salamanders Andrias davidianus (Anda-MHC) reveals novel splice variants. Developmental & Comparative Immunology, 2014, 42: 311-322
Zhu R, Chen ZY, Wang J, Yuan JD, Liao XY, Gui JF, Zhang QY. 2014. Thymus cDNA library survey uncovers novel features of immune molecules in Chinese giant salamander (Andrias davidianus). Developmental & Comparative Immunology. 46(2): 413-422
Chen ZY, Gui JF, Gao XC, Pei C, Hong YJ, Zhang QY. 2013. Genome architecture changes and major gene variations of Andrias davidianus ranavirus (ADRV). Veterinary Research. 44:101
He LB, Gao XC, Ke F, Zhang QY. 2013. A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation. Virus Research, 177194-200
Ke F, He LB, Zhang QY, 2013. Nonstructural protein NS80 is crucial in recruiting viral components to form aquareoviral factories. PLos One, 8(5): e63737
Li SH, Ou T, Zhang QY. 2013.Two virus-like particle cyanophages causing lytic infections in freshwater cyanobacteria Virologica Sinica. 28(5)303-305
Ou T, Li SH, Liao XY, Zhang QY. 2013. Cultivation and characterization of the MaMV-DC cyanophage that infects bloom-forming cyanobacterium Microcystis aeruginosa. Virologica Sinica. 28(5)266-271
Ou T, Zhu RL, Chen ZY, Zhang QY. 2013. Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus. Diseases of Aquatic Organisms. 106: 197-206
Zhu R, Wang J, Lei XY, Gui JF, Zhang QY. 2013. Evidence for Paralichthys olivaceus IFITM1 antiviral effect by impeding viral entry into target cells. Fish and Shellfish Immunology. 35:918-926
Gao EB, Gui JF, Zhang QY. 2012. A novel cyanophage with cyanobacterial non-bleaching protein a gene in the genome. Journal of Virology. 86(1):236-245
Lei XY, Tong Ou, Qi-Ya Zhang. 2012. Rana grylio virus (RGV) 50L is associated with viral matrix and exhibited two distribution patterns. PLos One. 7(8): e43033
Lei XY, Ou T, Zhu RL, Zhang. QY. 2012. Sequencing and analysis of the complete genome of Rana grylio virus (RGV). Arch Virol. 157:1559-1564
Li S, Sun F, Zhang YB, Gui JF, Zhang QY. 2012. Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway. PLos One. 7(3): e32427
Chen ZY, Lei XY, Zhang QY, 2012.The antiviral defense mechanisms in mandarin fish induced by DNA vaccine against a rhabdovirus. Veterinary Microbiology 157: 264-275
Lei XY, Chen ZY, He LB, Pei C, Yuan XP, Zhang QY. 2012. Characterization and virus susceptibility of a skin cell line from red-spotted grouper (Epinephelus akaara). Fish Physiol Biochem. 38(4):1175-1182
He LB, Ke F, Zhang QY. 2012. Rana grylio virus as a vector for foreign gene expression in fish cell. Virus Research. 163: 66-73
Pei C, Lei XY, Yuan XP, Wang D, Zhao QZ, Zhang QY. 2012. Herpes-like virus infection in Yangtze finless porpoise (Neophocaena phocaenoides): Pathology, ultrastructure and molecular analysis. Journal of Wildlife Diseases, 48 (1): 235-237
Ke F, He LB, Pei C, Zhang QY. 2011. Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site. BMC Genomics. 12:323
Zhu RL, Lei XY, Ke F, Yuan XP, Zhang QY. 2011. Genome of turbot rhabdovirus exhibits unusual non-coding regions and a novel additional ORF that could be expressed in fish cell. Virus Research. 155: 495-505
Kim YS, Ke F, Lei XY, Zhu R, Zhang QY. 2010. Viral envelope protein 53R gene highly specific silencing and iridovirus resistance in fish cells by amiRNA. PLoS One, 5(4): e10308
Kim YS, Ke F, Zhang QY. 2009. Effect of β-glucan on activity of antioxidant enzymes and Mx gene expression in virus infected grass carp. Fish & Shellfish Immunology. 27: 336-340
Gao EB, Yuan XP, Li RH, Zhang QY. 2009. Isolation of a novel cyanophage infectious to filamentous cyanobacterium Planktothrix agardhii (cyanophyceae) from Lake Donghu in China. Aquatic Microbial Ecology, 54:153-162
Zhao Z, Ke F, Shi Y, Zhou GZ, Gui JF, Zhang QY. 2009. Rana grylio virus thymidine kinase gene: an arly gene of iridovirus encoding for the cytoplasmic protein. Virus Gene, 38: 345-352
Ke F, Zhao Z, Zhang QY. 2009. Cloning, expression and subcellular distribution of a Rana grylio virus late gene encoding ERV1 homologue. Molecular Biology Reports, 36: 1651-1659
Zhu R, Zhang YB, Zhang QY, Gui JF. 2008. Functional domains and the antiviral effect of the dsRNA-dependent protein kinase PKR from Paralichthys olivaceus. Journal of Virology, 82: 6889-6901
Zhao Z, Shi Y, Ke F, Wei W, Gui JF, Zhang QY. 2008. Constitutive expression of thymidylate synthase from LCDV-C induces foci formation and anchorage-independent growth in fish cells. Virology, 372: 118-126
Tao JJ, Zhou GZ, Gui JF, Zhang QY. 2008. Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF. Virus Research, 132: 86-96
Zhou GZ, Gui L, Li ZQ, Yuan XP, Zhang QY. 2008. Generation and characterization of monoclonal antibodies against the flounder Paralichthys olivaceus rhabdovirus. Journal of Virological Methods. 148: 205-210
Zhao Z, Ke F, Li ZQ, Gui JF, Zhang QY. 2008. Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China. Archives of Virology, 153: 1143-1148
Chen ZY, Liu H, Li ZQ, Zhang QY. 2008. Development and characterization of monoclonal antibodies to spring viraemia of carp virus. Veterinary Immunology and Immunopathology, 123: 266-276
Lü AJ, Li ZQ, Zhang QY. 2008. Detection of cutaneous antibodies in excised skin explants from Grass carp, Ctenopharyngodon idellus, immune to Scophthalmus maximus rhabdovirus. Journal of Fish Diseases, 31: 559-565
Huang YH, Huang XH, Gui JF, Zhang QY. 2007. Mitochondria-mediated apoptosis induced by Rana glylio virus infection in fish cells. Apoptosis, 12(9): 1569-1577
Zhao Z, Ke F, Gui JF, Zhang QY. 2007. Characterization of an early gene encoding for dUTPase from Rana grylio virus. Virus Research, 123: 128-137
Tao JJ, Zhang QY, Gui JF. 2007. Isolation and characterization of a rhabdovirus from coinfection of two viruses in mandarin fish. Aquaculture, 262(1): 1-9
Huang YH, Huang XH, Zhang J, Gui JF, Zhang QY. 2007. Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocysis disease virus isolated in China. Viral Immunology, 20(1): 150-159
Zhou GZ, Li ZQ, Zhang QY. 2007. Establishment, characterization and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara). Marine Biotechnology, 9 (3): 370-376
Zhang QY, Tao JJ, Gui L, Zhou GZ, Ruan HM, Li ZQ, Gui JF. 2007. Isolation and characterization of Scophthalmus maximus (turbot) rhabdovirus. Diseases of Aquatic Organisms. 74: 95-105
Huang XH, Zhang QY. 2007. Improvement and observation of immunoelectron microscopic method for the localization of frog Rana grylio virus (RGV) in infected fish cells. Micron, 38(6): 599-606
Lü AJ, Dong CW, Du CS, Zhang QY. 2007. Characterization and inductive expression of flounder voltage-dependent anion channel gene (VDAC) in response to virus infection. Fish & Shellfish Immunology, 23: 601-613
Huang XH, Huang YH, Yuan XP, Zhang QY. 2006. Electron microscopic analysis of the viromatrix of Rana grylio Virus in a Fish Cell line. Journal of Virological Methods, 133(2): 117-123
Zhang QY, Zhao Z, Xiao F, Li ZQ, Gui JF. 2006. Molecular characterization of three Rana grylio virus (RGV) isolates and Paralichthys olivaceus lymphocystis disease virus (LCDV-C) in iridoviruses. Aquaculture, 251: 1-10
Liu YM, Zhang QY, Yuan XP, Li ZQ, Gui JF. 2006. Seasonal variation of virioplankton in a eutrophic shallow lake. Hydrobiologia, 560: 323-334
Liu YM, Yuan XP, Zhang QY. 2006. Spatial distribution and morphologic diversity of virioplankton in Lake Donghu, China. Acta Oecologica-International Journal of Ecology, 29: 328-334
Sun W, Huang YH, Zhao Z, Gui JF, Zhang QY. 2006. Characterization of the Rana grylio virus 3β-hydroxysteroid dehydrogenase and its novel role in suppressing virus-induced cytopathic effect. Biochemical & Biophysical Research Communications, 351: 44-50
Zhou GZ, Li ZQ, Zhang QY. 2006. Characterization and application of monoclonal antibodies against the turbot (Scophthalmus Maximus) rhabdovirus (SMRV). Viral Immunology, 19(4): 637-645
Zhang QY, Xiao F, Xie J, Li ZQ, Gui JF. 2004. Complete genome sequence of lymphocystis disease virus (LCDV-C) isolated from China. Journal of Virology, 78(13): 6982-6994