Education:

1996-2000: Fish molecular genetics in Institute of Hydrobiology, CAS , China.(Ph.D.)

1985-1988: Fish molecular genetics in Institute of Hydrobiology, CAS , China.(Master)

1981-1985: Genetics in Dept. of Biology, Wuhan University, Wuhan, China.(Bachelor)

Professional Experience:

2001-present: Professor, Institute of Hydrobiology, CAS , Wuhan, China.

1998-2001: Associate Professor, Institute of Hydrobiology, CAS , Wuhan, China.

1990-1998: Research Associate, Institute of Hydrobiology, CAS , Wuhan, China.

1988-1990: Research Assistant, Institute of Hydrobiology, CAS , Wuhan, China.

2002.6-2002.8: Visiting professor, National University of Singapore, Singapore.

1.Transgenic Fish Research

Faster-growing transgenic fish: The “all-fish” growth hormone gene construct was transferred into the fertilized eggs of common Carp, and fast-growing transgenic fish were successfully produced. The growth rate and feed conversion efficiency of F1 transgenic common carp are 42% and 11% higher over the control respectively.

Disease resistant transgenic fish: Using outer capsid protein VP7 gene of grass carp reovirus (GCRV) as target gene, and employing grass carp H1 promoter, a shRNA expression construct was constructed. Transferred this construct into the fertilized eggs of rare minnow, and obtained the GCRV resistant transgenic rare minnow.

2. Fish Genomics and Molecular Breeding Research

Construction of Libraries: Two BAC libraries of grass carp in the EcoRI and HindIII sites of the vector CopyControl pCC1BAC were constructed, providing a totally genome coverage of 14.2× haploid genome equivalents of grass carp. Two cDNA libraries from grass carp head kidney and spleen were also constructed.

EST sequencing: single pass sequencing on 10,000 randomly selected clones from grass carp head kidney cDNA library was performed, and 3074 unigenes were obtained.

Development of molecular markers: Over 1,000 SSR markers of grass carp were cloned, and SNP markers from immune-related genes were detected.

Linkage mapping and QTL mapping: In present, I am working on genetic linkage mapping of grass carp with SSR and SNP markers, and QTL mapping on growth rate, feed conversion efficiency and GCRV resistant traits.

1. Su J, Zhu Z, Wang Y, Jang S. Isolation and characterization of Argonaute 2: a key gene of the RNA interference pathway in the rare minnow, Gobiocypris rarus. Fish Shellfish Immunol, 2009, 26(1):164-170.

2. Su J, Yang C, Zhu Z, Wang Y, Jang S, Liao L. Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus). Fish Shellfish Immunol, 2009, 26(6):828-835.

3. Huang R, Gao LY, Wang YP, Hu W, Guo QL. Structure, organization and expression of common carp (Cyprinus carpio L.) NKEF-B gene. Fish Shellfish Immunol, 2009, 26(2):220-9.

4. Huang R, Sun XF, Hu W, Wang YP, Guo QL. Strcture, organization and expression of common carp (Cyprinus carpio L.) SLP-76 gene. Fish Shellfish Immunol, 2008, 24: 530-541.

5. Su Jianguo, Zhu Zuoyan, Wang Yaping, Zou Jun, Hu Wei. Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection. Immunogenetics, 2008, 60:195-205.

6. Su Jianguo, Zhu Zuoyan, Xiong Feng, Wang Yaping. Hybrid Cytomegalovirus-U6 Promoter-based Plasmid Vectors Improve Efficiency of RNA Interference in Zebrafish. Mar Biotechnol, 2008, 10:511-517.

7. Su Jianguo, Zhu Zuoyan, Wang Yaping, Xiong Feng, Zou Jun. The Cytomegalovirus Promoter-Driven Short Hairpin RNA Constructs Mediate Effective RNA Interference in Zebrafish In Vivo. Mar Biotechnol, 2008, 10:262-269.

8. Su J, Zhu Z, Wang Y. Molecular cloning, characterization and expression analysis of the PKZ gene in rare minnow Gobiocypris rarus. Fish Shellfish Immunol, 2008, 25(1-2):106-113.

9. Hu Wei, Li Shuangfei, Tang Bin, Wang Yaping, Lin Haoran，Liu Xiaochun, Zou Jun, Zhu Zuoyan. Antisense inhibition of two sGnRH genes expression suppresses GtH synthesis and gonad development in common carp Cyprinus carpio. Aquaculture, 2007, 271: 498-506.

10. Liu Weiyi, Yun Wang,Yao Qin, Ya-ping Wang, Zhu Zuoyan. Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre Recombinase Using a Combination of Mutant Lox Sites. Marine Biotechnology, 2007, 9(4): 420-428.

11. Wang Yun, Hu Wei, Wang Yaping, Liu Weiyi, Zhu Zuoyan. Identification and characterization of a novel splice variant of gonadotropin a subunit in the common carp (Cyprinus carpio). Journal of Fish Biology, 2007, 71: 1082-1094.

12. Liu Jing, Wang Na, Wang Yaping, Sun Yonghua, Zhu Zuoyan. Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6. Journal of Genetics, 2006, 85(3): 171-178.

13. Wang Na, Liu Jing, Wu Gang, Su Jiangguo, Wang Yaping, Sun Yonghua, Zhu Zuoyan. Knock down of gfp and no tail expression in zebrafish embryo by in vivo-transcribed short hairpin RNA with T7 plasmid system. J Biomed Sci, 2007, 14: 767-776.

14. Wang Na, Liu Jing, WangYaping, Sun Yonghua, Zhu Zuoyan. Molecular characterization of common carp (Cyprinus carpio) Sonic Hedgehog and discovery of its maternal expression. Dev Genes Evol, 2007, 217: 299-305.

15.  Hu Wei, Wang Yaping, Zhu Zuoyan. Progress in the evaluation of transgenic fish for possible ecological risks and its containment strategies. Science in China Series C, 2007, 50(5): 573-579.