Research
| Title： | Human inflammatory dendritic cells in malignant pleural effusions induce Th1 cell differentiation |
|---|---|
| First author： | Gu, Fei-fei; Wu, Jing-jing; Liu, Yang-yang; Hu, Yue; Liang, Jin-yan; Zhang, Kai; Li, Ming; Wang, Yan; Zhang, Yong-an; Liu, Li |
| Journal： | CANCER IMMUNOLOGY IMMUNOTHERAPY |
| Years： | 2020 |
| DOI： | 10.1007/s00262-020-02510-1 |
| Abstract： | Dendritic cells are crucial for the initiation and regulation of immune responses against cancer and pathogens. DCs are heterogeneous and highly specialized antigen-presenting cells. Human DCs comprise several subsets with different phenotypes and functional properties. In the steady state, human DC subsets have been well studied. However, the components of DC subsets and their immune functions during the inflamed setting are poorly understood. We identified and characterized DC subsets in the malignant pleural effusions of NSCLC patients. We analyzed the capacity of these DC subsets to induce T-cell differentiation. We observed the presence of inflammatory DCs (infDCs) and macrophages in the malignant pleural effusions of NSCLC patients, as identified by the CD11C(+)HLA-DR(+)CD16(-)BDCA1(+) and CD11C(+)HLA-DR(+)CD16(+)BDCA1(-) phenotypes, respectively. InfDCs represented approximately 1% of the total light-density cells in the pleural effusion and were characterized by the expression of CD206, CD14, CD11b, and CD1 alpha, which were absent on blood DCs. InfDCs also expressed CD80, although at a low level. As infDCs did not express CD40, CD83 and CD275, they remained functionally immature. We found that TLR agonists promoted the maturation of infDCs. Compared with macrophages, infDCs had a weaker capacity to phagocytose necrotic tumor cell lysates. However, only infDCs induced autologous memory CD4(+) T-cell differentiation into Th1 cells. For the first time, we found that infDCs were present in the malignant pleural effusions of NSCLC patients. We conclude that infDCs represent a distinct human DC subset and induce Th1 cell differentiation in the presence of TLR agonists. |
