Research
| Title： | Regain of sex determination system and sexual reproduction ability in a synthetic octoploid male fish |
|---|---|
| First author： | Lu, Meng; Li, Xi-Yin; Li, Zhi; Du, Wen-Xuan; Zhou, Li; Wang, Yang; Zhang, Xiao-Juan; Wang, Zhong-Wei; Gui, Jian-Fang |
| Journal： | SCIENCE CHINA-LIFE SCIENCES |
| Years： | 2020 |
| DOI： | 10.1007/s11427-020-1694-7 |
| Abstract： | Polyploids in vertebrates are generally associated with unisexual reproduction, but the direct consequences of polyploidy on sex determination system and reproduction mode remain unknown. Here, we synthesized a group of artificial octoploids between unisexual gynogenetic hexaploidCarassius gibelioand sexual tetraploidCarassius auratus. The synthetic octoploids were revealed to have more than 200 chromosomes, in which 50 chromosomes including the X/Y sex determination system were identified to transfer from sexual tetraploidC. auratusinto the unisexual gynogenetic hexaploidC. gibelio. Significantly, a few synthetic octoploid males were found to be fertile, and one octoploid male was confirmed to regain sexual reproduction ability, which exhibits characteristics that are the same to sexual reproduction tetraploid males, such as 1:1 sex ratio occurrence, meiosis completion and euploid sperm formation in spermatogenesis, as well as normal embryo development and gene expression pattern during embryogenesis. Therefore, the current finding provides a unique case to explore the effect of sex determination system incorporation on reproduction mode transition from unisexual gynogenesis to sexual reproduction along with genome synthesis of recurrent polyploidy in vertebrates. |
