Research
| Title： | IRF11 regulates positively type I IFN transcription and antiviral response in mandarin fish, Siniperca chuatsi |
|---|---|
| First author： | Li, Li; Chen, Shan Nan; Nie, P. |
| Journal： | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY |
| Years： | 2021 |
| DOI： | 10.1016/j.dci.2020.103846 |
| Abstract： | In vertebrates, a total of eleven interferon (IFN) regulatory factors (IRFs), IRF1 to IRF11 are reported, with the conserved presence of IRF1 to IRF9 in all classes of vertebrates. However, IRF10 has been reported only in fish and birds, and IRF11 seems to be a fish specific IRF member. In this study, IRF11 in mandarin fish Siniperca chuatsi was found upregulated following virus infection, and IRF11 was localized constitutively in nucleus as revealed through immunofluorescence test. The overexpression and/or luciferase reporter assays showed that IRF11 can induce transcriptionally the ISRE activity, and the expression of type I IFNs, IFNc and IFNh, as well as the IFN-stimulated gene, Mx, thus inhibiting the Siniperca chuatsi rhabdovirus (SCRV) replication as indicated in the reduced expression of virus protein genes. It is thus suggested that IRF11 in mandarin fish and probably in other teleost fish can exert its antiviral effect through the upregulation of type I IFNs and ISGs. |
