Research
| Title： | Single-Base Resolution Mapping Reveals Distinct 5-Formylcytidine in Saccharomyces cerevisiae mRNAs |
|---|---|
| First author： | Wang, Yafen; Chen, Zonggui; Zhang, Xiong; Weng, Xiaocheng; Deng, Jikai; Yang, Wei; Wu, Fan; Han, Shaoqing; Xia, Chao; Zhou, Yu; Chen, Yu; Zhou, Xiang |
| Journal： | ACS CHEMICAL BIOLOGY |
| Years： | 2022 |
| DOI： | 10.1021/acschembio.1c00633 |
| Abstract： | 5-Formylcytidine (f(5)C) is one type of post-transcriptional RNA modification, which is known at the wobble position of tRNA in mitochondria and essential for mitochondrial protein synthesis. Here, we show a method to detect f(5)C modifications in RNA and a transcriptome-wide f(5)C mapping technique, named f(5)C-seq. It is developed based on the treatment of pyridine borane, which can reduce f(5)C to 5,6-dihydrouracil, thus inducing C-to-T transition in f(5)C sites during PCR to achieve single-base resolution detection. More than 1000 f(5)C sites were identified after mapping in Saccharomyces cerevisiae by f(5)C-seq. Moreover, codon composition demonstrated a preference for f(5)C within wobble sites in mRNA, suggesting the potential role in regulation of translation. These findings expand the scope of the understanding of cytosine modifications in mRNA. |
