Research
| Title： | Regulating light, oxygen and volatile fatty acids to boost the productivity of purple bacteria biomass, protein and co-enzyme Q10 |
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| First author： | Peng, Lai; Lou, Wenjing; Xu, Yifeng; Yu, Siwei; Liang, Chuanzhou; Alloul, Abbas; Song, Kang; Vlaeminck, Siegfried E. |
| Journal： | SCIENCE OF THE TOTAL ENVIRONMENT |
| Years： | 2022 |
| DOI： | 10.1016/j.scitotenv.2022.153489 |
| Abstract： | Purple non-sulfur bacteria (PNSB) possess significant potential for bioresource recovery from wastewater. Effective operational tools are needed to boost productivity and direct the PNSB biomass towards abundant value-added substances (e.g., protein and co-enzyme Q10, CoQ10). This study aimed to investigate the impact of light, oxygen and volatile fatty acids (VFAs) on PNSB growth (i.e., Rhodobacter sphaeroides) and productivity of protein and CoQ10. Overall, the biomass yields and specific growth rates of PNSB were in the ranges of 0.57-1.08 g biomass g(-1) CODremoved and 0.48-0.71 d(-1), respectively. VFAs did not influence the biomass yield, yet acetate and VFA mixtures enhanced the specific growth rate with a factor of 1.2-1.5 compared to propionate and butyrate. The most PNSB biomass (1.08 g biomass g(-1) CODremoved and 0.71 d(-1)) and the highest biomass quality (protein content of 609 mg g(-1) dry cell weight (DCW) and CoQ10 content of 13.21 mg g(-1) DCW) were obtained in the presence of VFA mixtures under natural light and microaerobic (low light alternated with darkness; dissolved oxygen (DO) between 0.5 and 1 mg L-1) conditions (vs. light anaerobic and dark aerobic cultivations). Further investigation on VFAs dynamics revealed that acetate was most rapidly consumed by PNSB in the individual VFA feeding (specific uptake rate of 0.76 g COD g(-1) DCW d(-1)), while acetate as a co-substrate in the mixed VFAs feeding might accelerate the consumption of propionate and butyrate through providing additional cell metabolism precursor. Enzymes activities of succinate dehydrogenase and fructose-1,6-bisphosphatase as well as the concentration of photo pigments confirmed that light, oxygen and VFAs regulated the key enzymes in the energy metabolism and biomass synthesis to boost PNSB growth. These results provide a promising prospect for utilization of fermented waste stream for the harvest of PNSB biomass, protein and CoQ10. |
