Development of Leptolyngbya sp. BL0902 into a model organism for synthetic biological research in filamentous cyanobacteria----Institute of Hydrobiology, Chinese Academy of Sciences

Publications

Title：	Development of Leptolyngbya sp. BL0902 into a model organism for synthetic biological research in filamentous cyanobacteria
First author：	Gao, Hong; Wang, Yali; Huang, Ziling; Yu, Feiqi; Hu, Xi; Ning, Degang; Xu, Xudong
Journal：	FRONTIERS IN MICROBIOLOGY
Years：	2024
Volume / issue：	/
DOI：	10.3389/fmicb.2024.1409771
Abstract：	Cyanobacteria have great potential in CO2-based bio-manufacturing and synthetic biological studies. The filamentous cyanobacterium, Leptolyngbya sp. strain BL0902, is comparable to Arthrospira (Spirulina) platensis in commercial-scale cultivation while proving to be more genetically tractable. Here, we report the analyses of the whole genome sequence, gene inactivation/overexpression in the chromosome and deletion of non-essential chromosomal regions in this strain. The genetic manipulations were performed via homologous double recombination using either an antibiotic resistance marker or the CRISPR/Cpf1 editing system for positive selection. A desD-overexpressing strain produced gamma-linolenic acid in an open raceway photobioreactor with the productivity of 0.36 gm(-2)d(-1). Deletion mutants of predicted patX and hetR, two genes with opposite effects on cell differentiation in heterocyst-forming species, were used to demonstrate an analysis of the relationship between regulatory genes in the non-heterocystous species. Furthermore, a 50.8-kb chromosomal region was successfully deleted in BL0902 with the Cpf1 system. These results supported that BL0902 can be developed into a stable photosynthetic cell factory for synthesizing high value-added products, or used as a model strain for investigating the functions of genes that are unique to filamentous cyanobacteria, and could be systematically modified into a genome-streamlined chassis for synthetic biological purposes.