Research
| Name： | LUO Daji |
|---|---|
| Academic Title： | Professor |
| Education： | Ph.D. |
| Phone： | 027-68780278 |
| E-mail： | luodaji@ihb.ac.cn |
Resume：
Luo Lab has been focused on 3 major research areas: (1) Gene engineering in fish genetic breeding, (2) Genetic mechanism during sex reversal in fish, and (3) Regulation of gene expression, alternative splicing, non-coding RNAs and other elements during sex determination and sex differentiation.
Education:
09/2003 - 06/2008: Graduate student, Major in Genetics, College of Life Sciences, Wuhan University (Supervisor: Prof. Zuoyan Zhu)
12/2006 - 03/2007: Exchange Graduate Student, Helsinki University, Finland
07/2006 - 08/2006: Exchange Graduate Student of Summer School, Helsinki University, Finland
09/1999 - 06/2003: Undergraduate student, Major in Life Science, College of Life Sciences, Wuhan University
Research Experience:
06/2019 - : Full Professor, Institute of Hydrobiology, Chinese Academy of Sciences
11/2011 - 05/2019: Associate Professor (Tenured), Wuhan University
09/2016 - 09/2017: Visiting Scholar, University of California, San Diego, USA (Prof. Xiang-Dong FU, http://fugenome.ucsd.edu/)
09/2011 - 09/2012: Visiting Scholar, The Chinese University of Hong Kong, (Prof. Christopher Hon Ki Cheng)
05/2009 - 05/2013: Postdoctoral research on fish gene engineering in the laboratory of Zuoyan Zhu and Wei Hu, Institute of Hydrobiology, Chinese Academy of Sciences
09/2008 - 11/2011: Lecturer, Wuhan University
Professional Memberships:
1. Member of the Committee of China Zoological Society (Zebrafish branch)
2. Member of the Committee of China Society of Fisheries (Branch of Biotechnology and Genetics Breeding in Aquaculture)
3.Guest Editor, Special Issue “Sex Determination and Gonad Development: From Mechanism to Application” in Life-Basel
Research Support: (Active)
National Natural Science Foundation of China, Grant No 31922085 (PI), 01/01/2020-12/30/2022
National Natural Science Foundation of China, Grant No 31872191 (PI), 01/01/2019-12/30/2022
Natural Science Foundation of Hubei Province, China, Grant No.2020CFA056 (PI), 06/30/2020-06/30/2023
BR Project of the Chinese Academy of Sciences (PI), 01/01/2020-12/30/2022
Selected Publications：
1. Meng K, Lin X, Liu H, Chen H, Liu F, Xu Z, Sun Y, Luo D*. Gonadal bacterial community composition is associated with sex-specific differences in Swamp eels (Monopterus albus). Frontiers in Immunology. 2022; 13: 938326.
2. Chen H, Zhao Y, Chen K, Wei Y, Luo H, Li Y, Liu F, Zhu Z, Hu W, Luo D*. Isolation, identification, and investigation of pathogenic bacteria from Common Carp (Cyprinus carpio) naturally infected with Plesiomonas shigelloides. Frontiers in Immunology. 2022;13: 872896.
3. Liu F#, Qin Y#, Huang Y, Gao P, Li J, Yu S, Jia D, Chen X, Lv Y, Tu J, Sun K, Han Y, Reilly J, Shu X, Lu Q, Tang Z, Xu C*, Luo D*, Liu M*. Rod genesis driven by mafba in an nrl knockout zebrafish model with altered photoreceptor composition and progressive retinal degeneration. PLOS Genetics. 2022; 18:e1009841.
4. Zhao Y#, Chen K#, Liu F, Jiang M, Chen Z, Chen H, Song Y, Tao B, Cui X, Li Y, Zhu Z, Chen J*, Hu W*, Luo D*. Dynamic gene expression and alternative splicing events demonstrate co-regulation of testicular differentiation and maturation by the brain and gonad in Common Carp. Frontiers in Endocrinology. 2022;12: 820463.
5. Li Y#, Chen Z#, Liu H#, Li Q, Lin X, Ji S, Li R, Li S, Fan W, Zhao H, Zhu Z, Hu W*, Zhou Y*, Luo D*. ASER: Animal Sex Reversal Database. Genomics, Proteomics & Bioinformatics, 2021.19:873-881. (Cover story)
ASER：Animal Sex Reversal database，http://aser.ihb.ac.cn/
6. Chen H, Liu H, Li R, Lin X, Luo H, Ji S, Hu W, Luo D*. Blood cell identification and hematological analysis during natural sex reversal in rice field eel (Monopterus albus). Aquaculture 2021; 538.
7. Yu S#, Jiang T#, Jia D, Han Y, Liu F, Huang Y, Qu Z, Zhao Y, Tu J, Lv Y, Li J, Hu X, Lu Z, Han S, Qin Y, Liu X, Xie S, Wang QK, Tang Z, Luo D*, Liu M*. BCAS2 is essential for hematopoietic stem and progenitor cell maintenance during zebrafish embryogenesis. Blood. 2019; 133:805-815. (Cover story)
8. Zhou B#, Li X#,*, Luo D#, Lim DH, Zhou Y, Fu XD*. GRID-seq for comprehensive analysis of global RNA-Chromatin interactions. Nature Protocols. 2019; 14:2036-2068.
9. Luo D, Feng K, Zhu Z, Hu W*. Genrating gene knockout Oryzias Latipes and Rice Field eel using TALENs method. Methods in Molecular Biology. 2019; 1874:489-506.
10. Xiao R#,*, Chen JY#, Liang Z#, Luo D, Chen G, Lu Z, Chen Y, Zhou B, Li H, Du X, Yang Y, San KM, Wei X, Liu W, Lecuyer E, Graveley BR, Yeo G, Burge CB, Zhang QM, Zhou Y, Fu XD*. Pervasive Chromatin-RNA binding protein interactions enable RNA-based regulation of transcription. Cell. 2019; 178:107-121.
11. Han S#, Gao M#, Liu F, Xie S, Yu S, Lu Z, Hu X, Liu X, Qin Y, Qu Z, Huang Y, Lv Y, Tu J, Li J, Yimer TA, Tang Z, Luo D*, Chen F*, Liu M*. The knockout of USH2A in zebrafish causes hearing impairment and late onset rod-cone dystrophy. Human Genetics. 2018; 137: 779-794.
12. Qiu C, Cheng B, Zhang Y, Huang R, Liao L, Li Y, Luo D*, Hu W*, Wang Y*. Efficient knockout of transplanted green fluorescent protein gene in Medaka using TALENs. Marine Biotechnology. 2014; 16:674-83. (Cover story)
13. Liu Y#, Luo D#, Lei Y, Hu W*, Zhao H*, Cheng CH*. A high effective TALEN-mediated approach for targeted gene disruption in Xenopus tropicalis and zebrafish. Methods. 2014; 69:58-66.
14. Luo D, Hu W*, Chen S, Zhu Z. Critical developmental stages for the efficiency of somatic cell nuclear transfer in zebrafish. International Journal of Biological Sciences. 2011; 7: 476-486.
15. Luo D, Hu W*, Chen S, Xiao Y, Sun Y, Zhu Z*. Identification of differentially expressed genes between cloned and zygote-developing zebrafish embryos at the dome stage using suppression subtractive hybridization. Biology of Reproduction. 2009; 80: 674-684.
